![]() ![]() A422 maps to a loop (422-loop) that continues toward the phosphorylation sites. It was previously reported that A422V is less sensitive to dark pulse-induced phase resetting and has a reduced amplitude of the KaiC phosphorylation rhythm in vivo. We demonstrate here that the A422V KaiC mutant sheds light on the former mechanism. Neither the mechanism of KaiA-stimulated KaiC phosphorylation nor that of KaiB-mediated KaiC dephosphorylation is understood in detail at present. KaiA dimer stimulates phosphorylation of KaiC hexamer at two sites per subunit, T432 and S431, and KaiB dimers antagonize KaiA action and induce KaiC subunit exchange. We found that the Synechococcus elongatus KaiA, KaiB, and KaiC proteins in the presence of ATP generate a post-translational oscillator that runs in a temperature-compensated manner with a period of 24 h. [Vanderbilt Univ., Nashville, TN (United States) [Vanderbilt Univ., Nashville, TN (United States) Johnson, Carl H. [Vanderbilt Univ., Nashville, TN (United States) Xu, Yao [Vanderbilt Univ., Nashville, TN (United States) Mori, Tetsuya [Vanderbilt Univ., Nashville, TN (United States) Smith, Jarrod A. ![]() Loop-Loop Interactions Regulate KaiA-Stimulated KaiC Phosphorylation in the Cyanobacterial KaiABC Circadian ClockĮnergy Technology Data Exchange (ETDEWEB)Įgli, Martin [Vanderbilt Univ., Nashville, TN (United States) Pattanayek, Rekha [Vanderbilt Univ., Nashville, TN (United States) Sheehan, Jonathan H.
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